Journal: Frontiers in Oncology

Article Title: Influence of EGF and pro-NGF on EGFR/SORTILIN interaction and clinical impact in head and neck squamous cell carcinoma

doi: 10.3389/fonc.2023.661775

Figure Lengend Snippet: Sortilin decreases overall survival in HNSCC but nor EGFR/Sortilin complexes. (A) Examples of labeling performed on TMA slides. Two tumors with 4 different stains were compared: simple IHC staining for proNGF, Sortilin and EGFR and Duolink Brightfield ® PLA staining for EGFR/Sortilin interaction. Photographs taken under EVOS M5000 light microscope (x20). (B) Kaplan-Meier survival curve over 5 years as a function of the intensity of HRP immunohistochemical stainin of Sortilin (in red = strong staining; in black = weak or absent staining). The vertical lines represent the censored data (patients lost to follow-up or follow-up less than 5 years). (C) (i) Kaplan-Meier 5-year overall survival curve as a function of Duolink Brightfield EGFR/Sortiline PLA labeling intensity (in red = strong labeling; in black = weak or absent labeling). The vertical lines represent the censored data (patients lost to follow-up or with less than 5 years of follow-up). Example of light microscopy (x20) of light/no (ii) and strong (iii) PLA EGFR/sortilin staining (acquisition by EVOS M5000 microscope). Scale bar: 50 μm.

Article Snippet: The TMA slides contained 96 head and neck tumor samples (20 benign and 28 malignant tumors, including 11 squamous cell carcinomas), each tumor being divided into 2 samples on the slide (BioChain ® , Cat. Z7020051, lot B508149).

Techniques: Labeling, Immunohistochemistry, Staining, Light Microscopy, Immunohistochemical staining, Microscopy

Journal: International Journal of Medical Sciences

Article Title: Identification and validation of Novel Estrogen Biosynthesis Biomarkers in Sinonasal Inverted Papilloma

doi: 10.7150/ijms.101753

Figure Lengend Snippet: Schematic workflow for the identification and verification of biomarkers for sinonasal inverted papilloma (SNIP).

Article Snippet: Tissue microarray (TMA) slides containing SNIP, sinonasal malignancy, and control tissue samples were procured from SuperBioChips Laboratories (NH1001a; Seoul, Republic of Korea).

Techniques:

Journal: International Journal of Medical Sciences

Article Title: Identification and validation of Novel Estrogen Biosynthesis Biomarkers in Sinonasal Inverted Papilloma

doi: 10.7150/ijms.101753

Figure Lengend Snippet: AKR1B10, CYP2C19, and CYP3A5 protein levels were specific to SNIP. Representative immunocytochemistry images of (A) SNIP tissue, sinonasal squamous cell carcinoma (SNSCC) tissue, and control tissue. (B) Relative fluorescence intensity of biomarkers in SNIP, SNSCC, and control tissues. Mann-Whitney U test was used to determine the significance. *p < 0.05; **p < 0.01. n.s, not significant.

Article Snippet: Tissue microarray (TMA) slides containing SNIP, sinonasal malignancy, and control tissue samples were procured from SuperBioChips Laboratories (NH1001a; Seoul, Republic of Korea).

Techniques: Immunocytochemistry, Control, Fluorescence, MANN-WHITNEY

Journal: Biomolecules

Article Title: Two-Dimensional N -Glycan Distribution Mapping of Hepatocellular Carcinoma Tissues by MALDI-Imaging Mass Spectrometry

doi: 10.3390/biom5042554

Figure Lengend Snippet: Comparison of Core Fucose Glycans Detected by Lectin Histochemistry and MALDI-IMS. A commercial HCC TMA (Biochain) containing 16 sets of tumor (2 cores each), and matched non-tumor adjacent (one core) tissues was profiled by MALDI-IMS , or stained by lectin histochemistry with a recombinant core fucose binding lectin, N224Q-rAAL . Four representative TMA core sections were selected for comparison. Lectin histochemistry indicated tumor cores with low (top panel, ( a , b )) or high (bottom panel, ( c , d )) staining. Additional staining images are provided in . The image for each lectin stained core was captured individually, and images in the panel are from a 4× magnification by light microscopy. The individual core images were re-grouped in the panel to match the MALDI-IMS image orientation. Shown in the MALDI images are four mono-fucosylated glycans: 1. Hex5dHex1HexNAc4 ( m / z = 1809.661); 2. Hex5dHex1HexNAc4NeuAc1 ( m / z = 2122.720 + 2Na); 3. Hex6dHex1HexNAc5 ( m / z = 2174.772); 4. Hex7dHex1HexNAc6 ( m / z = 2539.904).

Article Snippet: An HCC TMA slide (Catalog No.: Z7020059, Lot No.: B506168) was purchased from Biochain, Inc.

Techniques: Comparison, Staining, Recombinant, Binding Assay, Light Microscopy